| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Acidithiobacillus ferrooxidans | ACCATCATGCCGACCACA | AACATCTGCGCTGCGGTA | 58.92 | 59.74 | 271 | 133 |
100.00%
|
100.00%
|
| Primer 22 | Acidithiobacillus ferrooxidans | ACAACCCGACTTTGCGCA | CGCACGGTTGGCAATTGA | 60.52 | 59.36 | 285 | 132 |
100.00%
|
100.00%
|
| Primer 23 | Acidithiobacillus ferrooxidans | TGCGTGGCAAGCATAAGC | TGACCGCAAGCTCGATGA | 59.12 | 59.03 | 210 | 132 |
100.00%
|
100.00%
|
| Primer 24 | Acidithiobacillus ferrooxidans | TGCGTGGCAAGCATAAGC | ACCGCAAGCTCGATGATCC | 59.12 | 60.23 | 208 | 132 |
100.00%
|
100.00%
|
| Primer 25 | Acidithiobacillus ferrooxidans | CGGAGCGTGGATGAACTCA | AAAGCGTTCGTCTGCGGA | 59.78 | 59.97 | 218 | 132 |
100.00%
|
100.00%
|
| Primer 26 | Acidithiobacillus ferrooxidans | CTTTCCGGAGCGTGGATGA | AAAGCGTTCGTCTGCGGA | 59.78 | 59.97 | 223 | 132 |
100.00%
|
100.00%
|
| Primer 27 | Acidithiobacillus ferrooxidans | TTTCCGGAGCGTGGATGA | AAAGCGTTCGTCTGCGGA | 58.62 | 59.97 | 222 | 132 |
100.00%
|
100.00%
|
| Primer 28 | Acidithiobacillus ferrooxidans | CTCGTCAAGGTCCATGCCA | TGCGCACCACTTCGATGA | 59.70 | 59.66 | 184 | 132 |
100.00%
|
100.00%
|
| Primer 29 | Acidithiobacillus ferrooxidans | TGCTCGTCAAGGTCCATGC | TGCGCACCACTTCGATGA | 60.38 | 59.66 | 186 | 132 |
100.00%
|
100.00%
|
| Primer 30 | Acidithiobacillus ferrooxidans | TCGTCAAGGTCCATGCCA | TGCGCACCACTTCGATGA | 58.52 | 59.66 | 183 | 132 |
100.00%
|
100.00%
|